ESTIMATION OF THE HAEMOLYTIC ACTIVITIES OF CAMEL COMPLEMENT

Document Type : Original Article

Authors

1 Department of Microbiology, Fac. Vet. Medicine, Cairo University.

2 Department of Microbiology, Fac. Vet. Medicine, Cairo University

3 Department of Pathology, Animal Health Research Institute, Dokki.

Abstract

29 serum samples from apparently healthy Sudanese camels were screened for total hemolytic complement activity. Four groups of animals were categorized according to age and Sex into group I: males over 7 years; group 2: males between 4.5-5 years; group 3: females over 7 years and group 4: females between 4.5-5 years. In this work, the sensitized horse erythrocytes (SHRBCs) proved to be the optimal indicator System for the estimation of camel complement hemolytic activity (CH50) with a percentage of hemolysis ranged between 13.3 - 46.2. Sensitized sheep erythrocytes (SSRBCs) did not give any detectable absorbencies. Only undiluted camel sera showed reasonable percentages of hemolysis ranged between 44.5-76.6, as these sera in the diluted form could not show any readable absorbencies. The average level of CH50 units were ranging between 0.25-2.94 in group 1; 0.44-1.66 in group 2; 0.50-2.13 in group 3 and 2.1 in group 4. The total average of CH50 units in female's sera showed slightly higher CH50 values than those in male's sera, with average CH50 units of 1.176 and 0.989 respectively.

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