Some studies on probiotics on humoral and Cellular immunity in broiler chicken

Authors

1 Dept. of Microbiol, Facult, of Vet. Med. Cairo University

2 Dept. of Poult, Dis., Facult. of Vet, Med., Cairo University

3 Dept. of Immunol, Animal Health Res. Institute, Dokki, Giza, Egypt

Abstract

This study aimed to investigate the effect of monospecific commercial probiotics (pediococcus acidilactici) on the cellular and humoral immune response of broiler chickens for routine vaccination against New Castle disease (ND) and Avian influenza (AJ). A total of 160, one-day broiler chickens were divided into 4 equal groups; 40 chickens for each. Chickens of the group (1) served as a negative control. Chickens of group (2) were fed on commercial ration supplemented with probiotic (pediococcus acidilactici) 1 Kg / ton of ration. Chickens of the group (3) were fed on commercial ration supplemented with probiotic {1 Kg/ton and vaccinated with inactivated NDV and AI - H5N1 vaccines. Chickens of the group (4) were given inactivated ND and AI - H5N1 vaccine. The results showed that probiotics supplementation stimulates both humoral and cell-mediated immune responses. Chickens supplemented with probiotics and vaccinated showed a significant increase in HI titers against NDV at 5" weeks compared to vaccinated chickens and against (AI) at 21 days post-vaccination among groups. Lysozyme activity revealed a significant increase in probiotic chickens versus non-treated ones at 1 and 5" weeks of age. Chicken supplemented with probiotic (G2) showed significant increase in phagocytic percent of macrophage and index at 1°, 2™ and 5' weeks as compared to control. The protection rate against challenges with NDV reached 90 % in chickens supplemented with probiotics and vaccinated while chickens vaccinated only revealed a 60 % protection rate. It was concluded that probiotics (pediococcus acidilactici) proved to be able to implement humoral and cell- mediated immune response.

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