Cross- reactions of the monospecific Hydatid cyst sera with some helminths antigens in sheep

Authors

1 The Arab center for the studies of Arid zones and Dry Lands, Syria, Damascus

2 Faculty of Veterinary — Albaath University

3 Faculty of Science — Damascus University

Abstract

Echinococcus Granulosus is the causative agent of Hydatidosis, which is an important zyonotic discase with worldwide distribution. This study aimed to identify the cross-reaction of the hydatid cyst fluid antisera with antigens of Fasciola hepatica, Taenia hydatigena (CysticercusTenuicollis), T. thysaniezia, and Moniezia expansa by application of immunoblot test and dot ELISA techniques. Mono-specific serum was obtained by immunizing rabbits against the 54, 38, and 27 KDa antigens individually; and Sera were collected from rabbits before injecting the antigens and used as a negative control. The results showed that serum induced by bands which have molecular weight 54 KDa antigen reacted with antigens of CysticercusTenuicollis and Moniezia expansa, while, serum induced by band 38 KDa antigen Strongly reacted with antigens of 7.thysaniesia and Fasciola Hepatica. On the other hand, serum induced by band 27 KDa antigen reacted with antigens of 7. thysaniesia and Moniezia Expansa. However, there was no cross – reaction between antigens of Fasciola hepatica and Cysticercus tenuicoillis with antiserum band 27 KDa. It is concluded that there is an antigenic relation between Hydatid cyst fluid and different studied parasites. It is proposed that a subunit with a molecular weight of 38 KDa is a satisfactory antigen that would be used in serodiagnosis for detection of Aydatidosis antibodies in Syrian sheep.

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