RT-PCR amplification of VIA (3D) gene and protease (3C) using its primers. Legation of the PCR product with the cloning vector (using pET system). Expression of the re- combinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA.
SHAWKY, M., SABER, M., ABDEL RAHMAN, A., & EL-SONOUSI, A. (1999). CLONING AND EXPRESSION OF 3CD GENES OF FMDV. Veterinary Medical Journal (Giza), 47(3), 289-294. doi: 10.21608/vmjg.1999.370851
MLA
M SHAWKY; M SABER; A ABDEL RAHMAN; A EL-SONOUSI. "CLONING AND EXPRESSION OF 3CD GENES OF FMDV", Veterinary Medical Journal (Giza), 47, 3, 1999, 289-294. doi: 10.21608/vmjg.1999.370851
HARVARD
SHAWKY, M., SABER, M., ABDEL RAHMAN, A., EL-SONOUSI, A. (1999). 'CLONING AND EXPRESSION OF 3CD GENES OF FMDV', Veterinary Medical Journal (Giza), 47(3), pp. 289-294. doi: 10.21608/vmjg.1999.370851
VANCOUVER
SHAWKY, M., SABER, M., ABDEL RAHMAN, A., EL-SONOUSI, A. CLONING AND EXPRESSION OF 3CD GENES OF FMDV. Veterinary Medical Journal (Giza), 1999; 47(3): 289-294. doi: 10.21608/vmjg.1999.370851
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