PREPARATION AND EVALUATION OF IMMUNOFLUORESCENT CONJUGATE AGAINST FMD VIRUS

Document Type : Original Article

Authors

FMD Research Department. Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Abstract

A trial for the production and evaluation of an immunofluorescent conjugate against FMD virus infection in farm animals was conducted locally. Using serum of experimentally infected animals collected after 35 days post infection. This serum was treated with saturated ammonium suplhate for precipitation and isolation of g- globulins and estimation of total protein content. It was found that total protein content of serum used was 4 gm / 100 ml serum according to the protein content conjugation of the antibodies with fluorescein was applied in constant fluorescein / protein ratio. These labeled antibodies were purified and tested against free dye physiochemically for protein content, antibody specificity, labeling ratio and potency performance (titration). From the present study it was found that fluorochrome conjugate end point was 1/!6 used in the test proper against positive samples for FMD virus. It is evident from the results obtained that all collected samples were giving positive results of high specificity against the prepared conjugate. The prepared fluorescent conjugated antiserum proved its efficiency for the localization of antigen of FMD virus in tissue sections and smears; also it is considered as rapid, easy and economic reagent for direct FA test used in viral diagnosis.
 
 

Main Subjects

Veterinary Medical Journal (Giza)
Volume 47, Issue 2
April 1999
Pages 239-246

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