Fractionation of somatic Voxexara — vitulorwmn antigen was carricd out using Sepharose 4 B gel filtration technqiue. The obtained results indicated that 4 fractions were recovered . F1-F4. All fractions were tested for their antigenicity by agar gel diffusion and ELISA tests. ELISA values of F1 & F2 were relatively similar, while F4 had no any reactivity with agar gel diffusion and ELISA tests. Fraction 2 was usde as identified specific antigen. This anti evaluated for its diagnostic applicability against sera of infected and non infected buffalo-calves by ELISA test. The sensitivity of the test was 100% in diagnosis of confirmed infected calves. Moreover, this test could detect 38.96% of coprologically examined negative calves. It was noticed that the immune response was increased as the intensity of infection had increased.
DERBALA, A. (1999). THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES. Veterinary Medical Journal (Giza), 47(1), 89-96. doi: 10.21608/vmjg.1999.370634
MLA
A DERBALA. "THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES", Veterinary Medical Journal (Giza), 47, 1, 1999, 89-96. doi: 10.21608/vmjg.1999.370634
HARVARD
DERBALA, A. (1999). 'THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES', Veterinary Medical Journal (Giza), 47(1), pp. 89-96. doi: 10.21608/vmjg.1999.370634
VANCOUVER
DERBALA, A. THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES. Veterinary Medical Journal (Giza), 1999; 47(1): 89-96. doi: 10.21608/vmjg.1999.370634