Studies on the pathogenicity of Enterobacter sakazakii infection to 1-day-old specific pathogen-free chick

Authors

1 Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University

2 Department of Poultry Diseases, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt

3 Veterinary service division, Ministry of Defense

Abstract

Sixty specific pathogen-free (SPE) chicks were divided into 3 equal groups; 20 each. Chicks of the “1” group IM group were subcutaneously (s.c) infected; Chicks of the “2” group were intra-crop infected. Each chick was infected with 1 ml. of 24 hours Enterobacter sakazakii (E. sakazakii) broth culture containing 1.5% x108 viable microorganisms Chicks of 3rd were kept as non-infected control The chicks were reared and kept under observation for 10 days. The mortality rate in E. sakazakii intra-crop infected SPF chicks was (4/20) 20% in the 1st 24 hours post-infection and 12.5% in chickens during 10 days: with total deaths of 30%. The mortality rate in s.c injected SPF chicks with E. sakazakii was (4/20) 20% at the 1st 24 hours post-infection and 20% out of the remaining 10 chickens in 10 days, with a total mortality of 60%. E. sakazakii could be isolated from all dead chicks. Both s.c and intra-crop infections resulted in microscopic lesions in the liver, spleen, kidneys, and intestine, Shedding of E. sakazakii in dropping was found to be an intermittent shedding. Clinical signs on chicks hatched from infected eggs were dullness, depression, brownish diarrhea, and coughing. Postmortem yntitis, and distended gall bladder, congested hat infection with £. sleepy appearance, ruffled feathers, lesions are congested lung, air sacculitis, hey and inflamed kidney. The results of performance indicated E. sakazakii decreased body weight and feed conversion rate (FCR). ate pathogenicity of E. sakazakii to 1-day old SPF In conclusion, our findings indicate the economic and public health importance of chicks with bad impact on the performance, of E. sakazakii are still need more investigation.

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