MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES

MADBOULY, H; ABDEL-MONEIM, A; JR, J; LADMAN, B; ROFAEL, NANCY

doi:10.21608/vmjg.2002.369385

MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES

Document Type : Original Article

Authors

¹ Virology Department, Faculty of Veterinary Medicine, Cairo University, Beni-Seuf Branch, Egypt.

² Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717

³ Serum and Vaccine Research Institute, Abbasia, Egypt

10.21608/vmjg.2002.369385

Abstract

Three field infectious bronchitis virus (IBV) strains isolated from different governorates in Egypt were examined molecularly using reverse transcriptase polymerase chain reaction (RT- PCR) and S1 partial gene sequence. RT-PCR using universal primers was positive for the three isolates. S-1 sequence analysis was successfully performed with two isolates only and failed with the third one. SI analysis of the two isolates revealed that one isolate was identical (100% aminoacid and nucleotide similarities) to Egypt/BeniSeuf/01 of a newly discovered genotype. The other isolate showed close similarity to Dutch variants D274 (98% aminoacid similarity and 99.4 %nucleotide similarity) and D3896 (94% aminoacid similarity and 97.8 %nucleotide similarity). Both isolates showed considerable high divergence to H120 (29.8% amino acid divergence and 25.8% nucleotide divergence for isolate 1 and 26.9% amino acid divergence and 23.9% nucleotide divergence for isolate 2) and M41 (30.4% amino acid divergence and 25% nucleotide divergence for isolate 1 and 28.0% amino acid divergence and 22.6% nucleotide divergence for isolate 2) vaccines.

Main Subjects