TRIAL FOR PREPARATION AND EVALUATION OF LIVE ATTENUATED COMBINED AVIAN ENCEPHALOMYELITIS AND FOWL POX VACCINE IN COMPARISON WITH IMPORTED ONE N.A

Document Type : Original Article

Authors

1 Central Laboratory for Control of Veterinary Biologics, Abbasia, Cairo.

2 Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo.

3 Central Laboratory for Control of Veterinary Biologics, Abbasia, Cairo

Abstract

An experimental trial was carried out to prepare a combined attenuated vaccine against avian encephalomyelitis (AE) and fowl pox diseases. Monovalent live attenuated vaccine was firstly prepared against each disease on specific pathogen free (SPF) eggs. After titration, they were mixed together and lyophilized as a bivalent live Vaccine. Chickens were vaccinated with the locally prepared vaccine as well as the Imported one. Humoral immune response of the vaccinated chicks was evaluated by using enzyme linked immunosorbent assay (ELISA) for avian encephalomyelitis” was proposed by Van Roekel et al. lomyelitis and neutralization test (NT) for AE and fowl pox viruses. The prepared combined vaccine was found potent, safe and protective against challenge viruses.

Main Subjects