EFFICACY OF VACCINATION STRATEGY AGAINST LUMPY SKIN DISEASE WITH THE USE OF NEW GENERATION OF INACTIVATED VACCINE

MADBOULY, H; HUSSEIN, H; MOHAMED, FATEHIA; EL-DESAWY, OMYMA; SABER, M; SHALABY, M

doi:10.21608/vmjg.2002.369375

EFFICACY OF VACCINATION STRATEGY AGAINST LUMPY SKIN DISEASE WITH THE USE OF NEW GENERATION OF INACTIVATED VACCINE

Document Type : Original Article

Authors

¹ Department of Virology, Faculty of Veterinary Medicine, BeniSuef Branch, Cairo University

² Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt

³ Department of Virology, Animal Health Research institute, Dokki Giza-Egypt.

⁴ Department of Virology, Animal Health Research institute, Dokki Giza-Egypt

10.21608/vmjg.2002.369375

Abstract

Lumpy skin disease (LSD) local strain (Ismalia 88) was propagated in MDBK cell line for six serial passages to obtain a titer of 107.5 /ml TCID50. Harvestation of the adapted virus from inoculated cell culture was based on the time at which a clear CPE was observed (5 days post inoculation). The virus was inactivated using binary ethyleneimine (BEI) at concentration of 2% for 12 hours. The inactivated virus was used with new generation of adjuvant (Nigella sativa oil) in the preparation of inactivated LSD local vaccine. The prepared vaccine was characterized using different techniques including the drop test, emulsion viscosity and stability. In addition, it was tested for the sterility and safety. Twenty Friesian calves (7 month-old) divided into 4 groups were inoculated with the different vaccination proGrammes as follow: group I was vaccinated with 2 doses of sheep pox vaccine with 6 months interval; group 2 was vaccinated with sheep pox vaccine and boostered after 6 months by the prepared LSD inactivated vaccine; group 3 was vaccinated with 2 doses of prepared inactivated LSD vaccine with 6 months interval and group 4 was left non vaccinated calves as negative control. Sera were collected monthly post vaccination for 8 months. The sera were tested by virus neutralization test and ELISA to evaluate the immune response of different vaccination strategies. The results of the in Vivo experiment revealed that the inactivated LSDV adjuvated with Nigella sativa oil was sterile, safe and elicited hight antibody response against LSD either when used for priming or boostering of animals alone or in combination with sheep pox vaccine.

Main Subjects