TRIALS FOR PREPARATION AND EVALUATION OF PEROXIDASE AND FLUORESCENT ISOTHIOCYANATE CONJUGATED ANTISERA AGAINST EQUINE INFLUENZA VIRUS

Document Type : Original Article

Authors

1 Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo.

2 Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Abstract

The present study was designed to produce antiinfluenza antibodies conjugated by peroxidase and fluorescein isothiocyanate separately for rapid detection of equine influenza (El) virus. Five rabbits were immunized with equine influenza (El) virus (A/equi/2) and one was kept as control. Immunoglobulins were separated using concentrated ammonium sulphate, the globulin content was quantitated and it was 2.5 gm/dl. These globulins were divided into 2 portions, the 1st one was conjugated with peroxides and gave a strong positive result when diluted 1:2000. The 2nd portion was conjugated with fluorescein isothiocyanate and gave a strong positive result when diluted 1:20. Both conjugates were tested and compared with an imported one on allantoic fluids previously proved to be positive to equine influenza and gave a strong positive reaction. The two prepared labeled antibodies could be used successfully in detection of influenza antigen during the course of the disease.

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