BIOLOGICAL AND IMMUNOLOGICAL CHARACTERIZATION OF ESCHERICHIA COLI PILI ISOLATED FROM CASES OF URINARY TRACT INFECTION IN HORSES

Document Type : Original Article

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Bacteriology Department, Animal Health Research Institute, Doki, Giza

Abstract

The bacteriological examination of 126 urine samples from horses with urinary tract infection revealed the isolation of E. coli from 76 cases (60.3%); Streptococcus spp. 14 cases (11.1%); Klebsiella spp. 12 cases (9.5%); Proteus spp. 11 cases (8.7%); Staphylosoccus spp. 7 cases (5.6%) Corynebacterium 3 cases (2.3%) and 3 cases were bacteriologically negative (2.3%). The incidence of E. coli isolation from mares was higher (63.9%) than stallions (55.6%). Three E. coli serogroups were identified 0:16, (26 isolates 20.6%), 0:2, (20 isolates 15.9%), and 0:6 (16 isolates 12.7%). The SDS-PAGE analysis of the pur- ified pili preparations from each serogroup revealed one distinct protein band 20.1kDa for 0:16 and 0:6, whereas a 16.6kDa band serogroup for the serogroup 0:2. The hemagglutintion activity of E. coli isolates to the different erythrocyte types was similar to their corresponding purified pili preparations. The effect of D-mannose on the hemagglutination of different erythrocytes revealed two types of hem- agglutination, the mannose sensitive (pili type I) and the mannose resistant hemagglutination (pili type II). In the in-vitro adherence test of E. coli isolates to urinary tract epithelial cells (UTEC), all E. coli isolates were highly adherent, especially the isolates of the serogroup 0:16. In the adhesion inhibition test using the homologus antipili antisera. The number of E. coli adhered to UTEC was significantly (P<0.01) reduce, whereas the hetrologus antibodies could reduce the adhesion in case of isolates of the serogroup 0:16 and 0:6 only. While heterologus antipili antisera could not inhibit the adhesion of the isolates from the

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