Document Type : Original Article
Authors
1
Dept. of Anim. Med., Fac. Vet. Med., Zagazig University, Benha Branch, Egypt
2
Dept. of Hygiene, Animal Behaviour and Management, Fac. Vet. Med.. Zagazig University, Benha Branch, Egypt.
3
Veterinary serum and vaccine Research Institute, Abbasia, Cairo, Egypt
Abstract
This study was carried out on a total number of 672 animals (551 cows and 121 calves), belonging to two private farms at Sharkia governorate, 352 of them showed an apparent clinical manifestations refereed to bovine ephemeral fever. The disease was sweeped throughout Egypt during the period from April to July 2000.
Virological examination revealed that, buffy coat samples obtained from viremic cattle on inoculation into BHK cell cultures elicited cytopathic effect (CPE) while in suckling mice resulted in appearance of neurological signs and subsequent death. The detected virus was confirmed by per- forming virus neutralization test. Meanwhile examination of buffy coats samples collected from incontact animals (buffaloes, sheep, goats, dogs And donkeys) revealed negative findings. Serological investigation declared that, infected and convalescent cattle had a significant titer of serum neutralizing antibodies while it did not de- tected in the sera of incontact animals except buffaloes had serum neutralizing antibodies without clinical evidence of the disease. Hematological panel of the infected cows showed anemia associated with leukocytosis and neutro- philia with lymphopenia. Serum biochemical pro- file revealed significant alteration in the values of the total protein, albumin, globulin, calcium, phosphorus, ALT, AST, creatinine, urea, copper, iron and zinc. The complicated cases declared pulmonary and subcutaneous emphysema broncho-pneumonia. Abortion and prolonged recumbency. The percent- ages of subclinical mastitis and reduction of milk
Main Subjects
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