Study on fungal contamination of some chicken meat products with special reference to the use of PCR for its identification

Aziz, Shaltout; Mahmoud, El-diasty; El-mesalamy, Manal; El-shaer, Manal

doi:10.21608/vmjg.2014.368822

Study on fungal contamination of some chicken meat products with special reference to the use of PCR for its identification

Document Type : Original Article

Authors

¹ Department of Food Hygiene, Faculty of Vet. Med. Moshtohor Benha University

² Department of Mycology, Animal Health Research Institute Dokki, Giza

³ Zagazig Provincial Lab, Department of Mycology, Animal Health Research Institute

⁴ Zagazig Provincial Lab, Department of Food Hygiene, Animal Health Research Institute

10.21608/vmjg.2014.368822

Abstract

This work was carried out to evaluate the fungal contamination of chicken meat products sold in local markets as well as identification of some isolated moulds using PCR technique. For identification of the isolated moulds, samples were subjected to mycological examination using the morphological (macroscopic and microscopic) characterization. Molecular identification using (ITS) was carried out for isolated Aspergillus and Penicillium species. The average total mould counts in the examined samples of chicken luncheon, chicken pane and chicken minced meat were 3.1 x 10²±0.82 x 10², 7.4x 10²± 5.4 x 10²and 1.7 x 10² ± 0.16 x 10²cfu/gm, respectively. In the examined samples, 9 mould genera were identified. The identified mould genera were Aspergillus, Eurotium, Penicillium, Geotrichum, Fusarium, Cladosporium, Mucor, Eupencillium and Acremonium species. The isolated species of Aspergillus parasiticus and Penicillium purpurogenum were subjected to PCR identification, and were sequenced in both directions. Sequences were analysed and aligned by Clustal method using the program of DNAstar (Laser-gene, Wisconsin, USA).

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