A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat

Hassan, Omaima; Tag El -Din, Hannan; Ahmed, Nashwa; Salem, Ghada

doi:10.21608/vmjg.2014.368819

A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat

Document Type : Original Article

Authors

¹ chemistry Department, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.

² Food Hygiene Department, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.

10.21608/vmjg.2014.368819

Abstract

The increased public concern and the launch of legislative Strategies within the framework of method validation and risk evaluation induced the need for the development of highly sensitive and specific analytical methods for the determination of steroid hormones in edible matrices. In this study, three extraction techniques of testosterone residues are tested, the manufacturer procedure described by the ELISA kits for testosterone in tissues, the method stated by Umberger et al. (1958) without or with cleanup. Extraction followed by estimation of testosterone residue by ELISA kits either for tissue or serum. Fifty imported meat samples were collected randomly from markets; each was subjected to six tested treatments. The cost of chemicals, materials and kits of each treatment was computed to be compared economically. The obtained results indicated that, there is a significant testosterone residue increase in the extraction techniques stated by Umberger et al., (1958). Also, clean up procedure has no significant effect. Moreover, there is no significant difference between commercial ELISA kits for tissues and that for serum the most expensive treatment was that described by manufacturer of r- Biopharm Kit. On the other hand the cheapest treatment was the extraction according to Umberger et al., (1958) followed by detecting testosterone residues using commercial kit for estimation of testosterone in serum. Taking into consideration both significance and economical points of view it is recommended to use Umberger et al., (1958) for extraction of meat testosterone and commercial ELISA kit for serum to detect testosterone residue in the extract.

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