Influence of Some Acidifiers on Pharmacokinetics and Tissue Residues of Amoxicillin in Healthy and Experimentally E. coli Infected Broiler Chicken

M, Mohammed; H, Youssef; A, El-Banna

doi:10.21608/vmjg.2014.368800

Influence of Some Acidifiers on Pharmacokinetics and Tissue Residues of Amoxicillin in Healthy and Experimentally E. coli Infected Broiler Chicken

Document Type : Original Article

Authors

¹ a Residues Analysis Unit, Reference Laboratory For Veterinary Quality Control On Poultry Production, Animal Health Research Institute, PO Box 12618, Dokki, Giza, Egypt

² Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, PO Box 12211, Giza, Egypt.

10.21608/vmjg.2014.368800

Abstract

This work investigated the influence of some acidifiers (Gallimix) on the disposition kinetics and tissue residues of β-lactam antibiotic (Amoxicillin) in healthy and experimentally infected broiler chicken with pathogenic E. Coli O78.Thebacterial colony count was also investigated in experimentally infected broiler chickens in the presence of acidifier alone as well as in the presence of acidifier and Amoxicillin together. The concentrations of Amoxicillin in serum were measured using reversed phase high performance liquid chromatography (HPLC) on samples collected at frequent intervals after drug administration. There was no significant increase in serum concentrations of Amoxicillin at 24 hours post administration however there was very high significant increase in serum concentrations at 48 hours in the group of birds treated with Gallimix as compared with the group given Amoxicillin alone in normal healthy broiler chicken. Amoxicillin was somewhat rapidly absorbed in infected chickens (t_1/2b1.605 ± 0.05 h) as compared with treated only with amoxicillin (t_1/2b1.622 ± 0.04 h) and treated with Gallimix (t_1/2b1.682 ± 0.05h). There was no significant increase in Amoxicillin concentration in examined tissues when compared with values at corresponding time intervals in normal healthy broiler chickens received acidifier (Gallimix). There is a gradual decrease in the colony forming unit (CFU) in infected chickens that was treated with Amoxicillin orally. (single and multiple doses (20 mg/kg b.wt.) and supplemented by the acidifier (Gallimix) as compared with infected non-treated control group.

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