Some biochemical and immunomodulatory effects of marbofloxacin in sheep vaccinated with live sheep pox vaccine

Anwar, Abeer; K, Ghattas; Attia, Taha

doi:10.21608/vmjg.2011.368355

Some biochemical and immunomodulatory effects of marbofloxacin in sheep vaccinated with live sheep pox vaccine

Document Type : Original Article

Authors

¹ Immunology Unit, Animal Reproduction Research Institute

² Biology Department Animal Reproduction Research Institute

³ Pharmacology Dept., Faculty of Vet. Med., Menofiya University, Sadat Branch

10.21608/vmjg.2011.368355

Abstract

The study was performed to investigate de effect of marbofloxacin (2 mg/kg body weight/day) on some immunological parameters in 9 sheep (35-40 kg body weight) immunized with live attenuated sheep pox vaccine. sheep were allocated into 3 equal groups. The first group was left as control; the second group was injected intramuscular (I/M) by marbofloxacin (2mg/kg body weight/day for 5 successive days) then vaccinated by intradermal (I/D) injection of 0.5 ml sheep pox vaccine/sheep. The third group was vaccinated with sheep pox vaccine only.
The data revealed stimulation in the level of superoxide anion produced by neutrophil represented by detection of cytochrom C reduction level in the 2nd group (p<0.05) on the 1st day post vaccination, while there was significant depression in the level of lysozyme in the 1st two days post vaccination. The level of serum nitric oxide was not affected in the three groups. The lymphocytes transformation depressed significantly (p< 0.05) in the 1st week post vaccination in the 2nd group compared with the other two groups. It was concluded that marbofloxacin in its therapeutic level induced initial immunostimulant effect on the neutrophil followed by a transient immunosuppressive effect on other cells as macrophage when used before vaccination with live vaccine.

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