Evaluation of Redox Status, Clinicopathological Parameters and Cytogenetic Changes in Dairy Cattle Exposed to AflatoxinB1 and Zearalenone Contaminated ration

Document Type : Original Article

Authors

Animal Reproduction Research Institute, Giza, Egypt. Department of Biology.

Abstract

The objective of this study was to evaluate the effect of aflatoxin B1 and zearalenone on oxidant / antioxidant balance, liver and kidney functions in addition to chromosomal abnormalities in dairy cows.   A total number of 33 dairy muliparous Holstein Friesian lactating cows were under investigation, their body weight ranged from 650-750 kg. Cows were classified into two groups, control group (n=11) received ration where aflatoxin B1 and zearalenone concentrations not exceed the permissible limits. While the other group (n=22) fed ration contaminated with aflatoxin B1 and zearalenone mycotoxins. Three blood samples were taken from each cow . 
The first used for serum separation and the obtained sera were used for determination of copper and zinc levels, liver enzymes (ALT- AST), urea and creatinine. The second sample used for plasma separation and estimation of enzymatic antioxidant activities (glutathione peroxidase, superoxide dismutase and catalase) and malondialdehyde. While the third blood sample used for cytogenetic analysis. The obtained results of the second group showed that there were significant imbalance in most of oxidant / antioxidant parameters (significant decrease in glutathione peroxidase, superoxide dismutase and catalase on contrary a significant increase in malondialdehyde). Copper and zinc showed statistically non significant decrease. Additionally, liver enzymes and urea showed significant increase while statistically non significant decrease recorded in the level of serum creatinine. Concerning the cytogenetic changes, the same group revealed significant increase in numerical aberrations (peridiploidy) and structural aberrations (deletions, fragments) in addition to gonosomal anomalies (59-ΧΟ). Generally, the total percent of aberrated cells was significantly increased. In conclusion, aflatoxin B1 and zearalenone contaminated ration cause a harmful effects on redox status, liver function, in addition to their adverse effect on chromosomal abnormalities which reflect negatively on farm production and economy.

Main Subjects

Files

Share

How to cite

Statistics