Embryo Recovery, Characterization and Progesterone Level as affected by Oxytocin, Prostaglandin F₂α Treatments in rabbit.

Document Type : Original Article

Authors

1 Department of Poultry Production, Faculty of Agriculture, Mansoura University, Egypt.

2 Department of Animal Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt.

Abstract

  This study was carried out to investigate the various effected of intrauterine cavity injection just after mating with prostaglandin F₂α (33.3µg/kg) and oxytocin (OT) 1.6 IU/kg on embryo recovery, characterization and progesterone concentration in rabbit. There are significant differences (P<0.05) in ovulation rate between groups, the does that receiving oxytocin, PGF2α exhibited the highest number of corpora lutea (CL) than control group 13.0, 9.0 and 8.3 respectively.
    The percentage of embryos recovered was higher in PGF2α treated group 70.4% followed by Oxytocin group 56.4%, than control group 36.0 associated with no significant variations between treatments in the percentage of visible abnormal embryos as detected by degeneration of mass cell or embryos having irregular morphology. Average measurements for embryo dimension were studded and found that, zona pellucida (ZP) thickness of embryos recovered was significantly (P<0.05) thickes in oxytocin treated 26.7 µm compared with control 24.1 µm and PGF2α 20.6 µm. Progesterone concentration at 24hr were significantly higher and sharply increased at 48hr to reach nearly 3 time higher than control group. In conclusion injection rabbit does with PGF2α or oxytocin, increased ovulation rate and embryos recovery, which may increase does raising profitability in term of larger litter size.

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