Preparation of Combined Equine Influenza and Tetanus Toxoid Vaccine from local stains

Document Type : Original Article

Authors

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo

Abstract

   Inactivated equine influenza from local isolate and tetanus toxoid (EITT) vaccine adjuvanted with saponin was prepared. It was inoculated into Guinea pigs as a preliminary evaluation for its potency, which exceeds the permissible protective level allowed to be used for vaccination of horses. The vaccine proved to be safe and potent for horses and Guinea pigs. The mean haemagglutination inhibition (HI) antibody titres (3 weeks post vaccination) were 704 and 200 and the mean toxin neutralizing (TN) antibodies (2 weeks after boostering) were 37.9 and 45 IU/ml respectively. The antibody titre in vaccinated hones was monitored up to 6 months post re- vaccination. The keeping quality of the prepared vaccine was studied for one year. It was found to be stable when stored at 4°C.

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