Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals
Two models of ELISA were developed for serological detection of Bovine Ephemeral fever (BEF) antibodies in the sera of vaccinated Buffaloes in comparison with serum neutralization test. Blocking ELISA (B. ELISA) was developed and standardized for detection of BEF antibodies. In this test, the binding capacity of rabbit BEF hyper immune sera to BEFV antigen was blocked in the presence of positive BEF vaccinated Buffaloes sera. The sensitivity of both ELISA models was compared with serum neutralization test (SNT) using a total of 160 serum samples obtained from vaccinated Buffaloes where it was found that the sensitivity and specificity of B- ELISA and I-ELISA were (98.4% & not estimated) and (100% & 100%), respectively. These finding suggested that the both ELISA models were considered an inexpensive, rapid and sensitive serological methods that would be suitable for measuring of BEFV antibodies in vaccinated Buffaloes.
M, B., Mostafa, H., I, I., Mahmoud, N., M, A. E., & F, Z. (2011). Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals. Veterinary Medical Journal (Giza), 59(3), 19-25. doi: 10.21608/vmjg.2011.368297
MLA
Barghouth M; Hanna Mostafa; Ismail I; Nermeen Mahmoud; Abd El-Bakey M; Zaky F. "Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals", Veterinary Medical Journal (Giza), 59, 3, 2011, 19-25. doi: 10.21608/vmjg.2011.368297
HARVARD
M, B., Mostafa, H., I, I., Mahmoud, N., M, A. E., F, Z. (2011). 'Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals', Veterinary Medical Journal (Giza), 59(3), pp. 19-25. doi: 10.21608/vmjg.2011.368297
VANCOUVER
M, B., Mostafa, H., I, I., Mahmoud, N., M, A. E., F, Z. Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals. Veterinary Medical Journal (Giza), 2011; 59(3): 19-25. doi: 10.21608/vmjg.2011.368297
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