Characterization of Recombinant Baculovirus Expressing envelope glycoprotein E of the local BoHV-1

Document Type : Original Article

Authors

1 Veterinary Serum and Vaccine Research Institute, P.O.Box:131, Abbassia, Cairo.

2 Department of Virology, Faculty of Veterinary Medicine, Cairo University, 11221Giza, Egypt.

Abstract

 A recombinant baculovirus expressing envelope glycoprotein E (gE) of the local Bovine Herpesvirus type-1 (BoHV-1) "Abu- Hammad strain was characterized. PCR analysis was conducted on the genomic DNA of recombinant baculovirus to verify the presence of the inserted gE gene. Spodoptera frugiperda (Sf9) cells infected with the recombinant baculovirus expressed a high level of immunoreactive recombinant gE protein that was demonstrated by indirect immunoflurescence and Western blot analyses. The recombinant gE protein was used as coating antigen in an indirect ELISA for detection of BoHV-1 anti-gE antibody to differentiate cattle vaccinated with the local of BoHV-1 "Abu-Hammad strain" from those vaccinated with gE _negative marker vaccine. A limited number of bovine serum samples which previously tested by the indirect ELISA, virus neutralization Test (VNT) and commercial blocking gE ELISA (IDEXX) were used to be tested by indirect ELISA. The obtainted results proved the reactivity of the recombinant gE protein and its utility as a diagnostic antigen in a gE based indirect ELISA for diagnosis of BoHV-1 as well as distinguishing infected animals within a gE marker vaccinated herd.

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