Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test
Foot-and-mouth disease virus (FMDV) serotypes (0/1/3/93) and (A/Egy/2009), grown on BHK-21 clone 13 monolayer cell line, inactivated with Binary Ethylenimine (BEI) and adjuvanted with 30% Alhydragel [AL(OH)3] were used for preparation of bivalent gel adjuvant vaccine currently used in Egypt 146s was measured using sucrose density gradient ultracentrifugation and vaccine dose was estimated as 4.2 µg of 146s viral proteins for each vaccine serotypes. Evaluation and testing of FMD vaccine for safety, sterility and potency were carried out. Cattle protective dose 50% (PD50) was determined. The specific developed neutralizing antibody responses against different doses of bivalent vaccine in correlation with the challenge exposure test for serotype (01/3/93) virus that was done 21 days post vaccination were undertaken. The obtained potency of a vaccine with an overall 50% cattle protective dose (PD50) value was 9.99 (PD50) for serotype (O/1/3/93).
A, B., A, S., M, B., M, I., M, A. E. A., M, A., & Α, F. (2011). Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test. Veterinary Medical Journal (Giza), 59(3), 27-37. doi: 10.21608/vmjg.2011.368228
MLA
Bakr A; Saad A; Barghouth M; Ibrahim M; Abd El Aty M; Aly M; Farag Α. "Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test", Veterinary Medical Journal (Giza), 59, 3, 2011, 27-37. doi: 10.21608/vmjg.2011.368228
HARVARD
A, B., A, S., M, B., M, I., M, A. E. A., M, A., Α, F. (2011). 'Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test', Veterinary Medical Journal (Giza), 59(3), pp. 27-37. doi: 10.21608/vmjg.2011.368228
VANCOUVER
A, B., A, S., M, B., M, I., M, A. E. A., M, A., Α, F. Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test. Veterinary Medical Journal (Giza), 2011; 59(3): 27-37. doi: 10.21608/vmjg.2011.368228
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