Occurrence of Enterobacter sakazakii in some meat products sold in Assiut governorate

M, Abd El-Malek; A, Ismail; S, Refaie

doi:10.21608/vmjg.2010.368189

Occurrence of Enterobacter sakazakii in some meat products sold in Assiut governorate

Document Type : Original Article

Authors

¹ Food Hygiene Department, Animal Health Research Institute, Assiut Regional Laboratory, Egypt

² Food Hygiene Department, Faculty of Veterinary Medicine; Assiut University

10.21608/vmjg.2010.368189

Abstract

A total number of 75 samples including 25 samples each of luncheon, minced frozen beef and sausage were collected from Assiut city (Upper Egypt) &examined bacteriologically for the presence of E. sakazakii and other Enterobacteriaceae genera& species by using the FDA enrichment procedure. In addition, a polymerase chain reaction (PCR) method for the rapid detection of E. sakazakii was adapted by using a set of primers designed from a region of the 16S ribosomal RNA gene. E. sakazakii was detected in 7 (28%) of sausage, but none of the luncheon and minced frozen beef samples were positive for E. sakazakii. Moreover, one false positive isolate was observed with FDA method. In contrast, PCR was highly sensitive and specific to E. sakazakii. E. cloacae were isolated from all samples, while E. agglomerans was detected only in luncheon samples. Also, 43 isolates related to family Enterobacteriaceae could be isolated including Citrobacter freundii, Escherichia coli, Hafnia alvei, Klebsiella pneumonia, K.oxytoca, K.ozaenae, K. planticola and Serratia marcescens. The public health importance of E. sakazakii and suggested control measures were discussed.

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