Incidence of bacterial contamination in wastes of private poultry life markets laughter premises in Sana’a city. I: Isolation, Identification and antibiogram of isolates.

This study was carried out to investigate the bacterial contamination and their drug sensitivity pattern in private poultry life markets and slaughter premises (shops) wastes in Sana’a city. Examination of collected 146 samples including intestinal contents (63), water from washing tanks (17),feathers (19), Spleen (25) and long bones (22) revealed 35,11,5,1 an 0 isolates in a rate of 55.5, 64.7, 26.3, 4 and 0%; respectively. The obtained bacterial isolates were subjected to colonial morphological, Gram stain reaction and biochemical identification. Bacteriological examination of samples revealed that 52 isolates were recovered in a total rate of 35.6%. These isolates were including 27 (51.9%) E. coli; 10 Citrobacter (19.3%); 3 (5.7%) Klebsiella Pneumoniae (K. Pneumoniae); 1(1.9%) Salmonella Enteritidis (S. Enteritidis); 3 (3.8%) Campylobacter jejuni (C. jejuni); 1 (1.9%) Pseudomonas aeruginosa  (Ps.aeruginosa); 5 Staphylococcus coagulase negative (9.6%); and 3 Streptococcus β haemolytic (5.7%). Results of sensitivity of bacterial isolates against used 16 antibiotics. It was observed that no single antimicrobial drug was 100% effective against different isolates. It is clear that most isolates were resistant to Tetracycline, Oxytetracycline, and Erythromycin. We recommend the strict application of sanitary measures in life poultry markets to minimize bacterial contamination and spread of pathogens; especially to human. The obtained isolates will be subjected to pathogencity test for 1-day-old chicks.