Genotypic Characterization of Antibiotic Resistant Escherichia coli Isolates Recovered from Baladi Chicks in Fayoum Governorate

Document Type : Original Article

Authors

1 Directorate of Veterinary Medicine, Fayoum, Egypt

2 Microbiology Department, Faculty of Veterinary Medicine, Cairo University

3 Poultry Diseases, Faculty of Veterinary Medicine, Cairo University

4 Department of Bacteriology, Animal Health Research Institute, Fayoum Laboratory, Agricultural Research Center, Egypt

Abstract

A total of 23 isolates of E. coli, recovered from Baladi chicks manifested respiratory signs associated with diarrhea and belonged to different O-serogroups was investigated for antimicrobial resistance and tested for their tetracycline and ciprofloxacin resistance genes. The result recovered that 91.3% of the isolates were resistant to tetracycline and 26.1% to ciprofloxacin (quinolone group). Ten out of 23 E. coli isolates tested for detection of antimicrobial resistance genes, 5 (50%) isolates were positive for tetA(A) gene, where 5 (50%) isolates were positive for tetA(B) gene. On the other hand all the E. coli isolates 10 (100%) were positive for gyrA gene, where only one isolate (10%) was positive for aac(6’)-Ib-cr gene.

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