A canine model of carbon tetrachloride (ccl4) induced liver cirrhosis: II.Ultrasonographic and histopathological study

Document Type : Original Article

Authors

1 Dept of Surgery, Anesthesiology and Radiology, Faculty of Vet Med, Cairo University, Egypt.

2 Dept of Medical Biochemistry and Molecular Biology, Faculty of Med, Cairo University, Egypt

3 Dept of Pathology, Animal Health Research Institute, Ministry of Agriculture, Egypt.

4 Dept of Surgery, Anesthesiology and Radiology, Faculty of Vet Med, Cairo University, Egypt

Abstract

Liver cirrhosis is a diffuse state with replacement of the normal lobular architecture by abnormal nodules and fibrous septa. The Laennec staging system subdivides cirrhosis into 3 groups (4A,4B,4C) based on the thickness of fibrous septa and the size of nodules. 
This study aimed to assess the degree of liver cirrhosis through ultrasonographical and histopathological study after the administration of CCl4, in order to establish a canine model of liver cirrhosis for further research work. Experimental induction of liver cirrhosis was carried on 18 skeletally mature mongrel dogs using carbon tetrachloride (CCl4) for 16 weeks; dogs were evaluated for cirrhosis using ultrasonographical examination with portal vein (PV) and hepatic vein (HV) diameter, histopathological changes every 4 weeks and finally postmortem examination. Liver fibrosis was subdivided through liver biopsy under the guidance of ultrasonography using the Laennec staging system. Liver cirrhosis was confirmed by abnormal liver ultrasononography with portal hypertension, liver cirrhosis on biopsy sample was scored 4A,4B,4C based on the thickness of the fibrous septa and the size of nodules according to Laennec staging system. The degree of liver cirrhosis was recorded through ultasonographical and histopathological examination.

Keywords

Main Subjects

Veterinary Medical Journal (Giza)
Volume 62, Issue 1
January 2016
Pages 27-34

Files

Share

How to cite

Statistics