Phenotypic Characterization and Genotypic Analysis of Salmonella Typhimurium Isolated from Egyptian Pigeons

S, Abdelhassib; O, Ahmed; A, Oda; R, ElSeedy; F, Hatem

doi:10.21608/vmjg.2016.363162

Phenotypic Characterization and Genotypic Analysis of Salmonella Typhimurium Isolated from Egyptian Pigeons

Document Type : Original Article

Authors

¹ General Organization for Veterinary Services

² Animal Health Research Institute

³ Beni Suef University, Bacteriology Department

⁴ Cairo University, Bacteriology Department

10.21608/vmjg.2016.363162

Abstract

Bacteriological findings of 102 examined cases from adult pigeons and squabs revealed that 4 out of 102 (3.9%) cloacal swab samples were positive for Salmonella species, while 16 drag swabs collected from pigeon houses and dovecotes were negative. Culture characters as well as the identical biochemical and serological tests identified Salmonella species. By using slide agglutination test, four Salmonella isolates from pigeons were serotyped using O and H poly, monovalent antisera, three (isolates were serotyped as Salmonella Typhimurium, and one isolate was un-typed. By using agar gel diffusion method, the. antimicrobial sensitivity test for Salmonella Typhimurium isolated from pigeons were sensitive to Amoxicillin, Ciprofloxacin, Doxycycline, Enrofloxacin, Gentamicin, Norfloxacin and Streptomycin, while they were resistant to Erythromycin. PCR technique was used to detect different virulent genes as invasion (invA) and flagellin (fliC) genes and the three isolates gave : positive to all previous genes. Two fliC sequences were submitted to GenBank database and obtained accession numbers {HSE-1-2013 (KJ700871) and HSE-2-2013 (KJ671550)}and showed 99.8 % identity with each other and different identity percent with 22 randomly selected strains from GenBank. strain HSE-1-2013 consists of 158 amino acid showed 100% identity with the second strain HSE-2-2013 and the twenty two randomly selected strains from GenBank, while strain HSE-2-2013 | consists of 159 amino acid showed 100% identity with Egyptian strains (Azharl,2,3, and 4) and 99.5% identity. Other fifteen strains on the GenBank (there is protein changed at position! 59, the standard amino acid V Valine mutated to the amino acid E | Glutamic acid). The restriction enzyme map was constructed for the two sequenced PCR products of Salmonella Typhimurium and the enzyme Bbvl was found to cut five times at the positions 134,361,418,441 and 457.

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