Histopathological and clinicopathological studies on the antioxidant effect of gold nanoparticles in diethyInitrosamine induced hepatocarcinogenesis in rats

Document Type : Original Article

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Department of Pathology, Faculty of Veterinary Medicine, Cairo University

Abstract

This study investigated the antioxidant and therapeutic effect of gold nanoparticles (GNPs) against diethylnitrosamine (DENA) induced hepatocarcinogenesis. 24 rats were divided into four groups. The first group kept as a negative control group without any treatments. The second group received single intraperitoneal injection of DENA (200 mg/kg) followed by weekly subcutaneous injections of carbon tetrachloride (3 ml/kg) for 10 weeks and kept as a positive control group. The third group was intoxicated by DENA and CCLA as the second group then treated by I.p. Injection of 0.075 mg free GNPs kg-1 twice (at day, 0 and 3) then retreated with the same dose after 21 days. The fourth group received the same dose of GNPs as in third group. The results showed that group injected with DENA expressed high levels of lipid peroxidation (MDA) accompanied by significantly decreased levels of antioxidants (reduced glutathione and Catalase) when compared with the control group, on the other hand there was significant decrease in MDA and Increase in antioxidants in GNPs treated group. Concomitantly, the improvement of histopathological alterations induced by DENA in livers of GNPS treated groups was observed. In conclusion, these results suggested a positive hepatotherapeutic and antioxidant effect of GNPs by decreasing lipid peroxidation and enhancing the levels of antioxidants in DENA-induced hepatocarcinogenesis by reducing the formation of free radicals.

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