This study aimed to compare the efficiency of dimethyl-formamide (DMF), glycerol and their combination on freezability of Arabian stallion spermatozoa. Fourty semen samples were collected from four Arabian stallions, diluted with INRA-82 supplemented with 5% glycerol, 5% DMF or 2.5% glycerol+2.5% DME and subjected to cryopreservation. The results revealed the superiority of DMF vs. glycerol in terms of post-thaw motility (56.25 ± 0.89 vs. 46.50 ± 1.14%, respectively), viability index (167.75 ± 3.49 vs. 135.50 ± 4.49, respectively), survival rate (74.58 ± 1.03 vs. 61.59 ± 0.38%, respectively) and sperm acrosome integrity (78.83 ± 0.74 vs. 75.95 ± 0.74%, respectively). In conclusion, The protocols of Arabian stallion sperm cryopreservation could be enhanced using DMF as a cryoprotectant (CPA) used with INRA-82 extender.
Fadl, A., A, E., M, A. A., & M, G. (2016). Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa. Veterinary Medical Journal (Giza), 62(2), 59-63. doi: 10.21608/vmjg.2016.363158
MLA
Aya Fadl; El-Badry A; Abou Ahmad M; Ghallab M. "Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa". Veterinary Medical Journal (Giza), 62, 2, 2016, 59-63. doi: 10.21608/vmjg.2016.363158
HARVARD
Fadl, A., A, E., M, A. A., M, G. (2016). 'Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa', Veterinary Medical Journal (Giza), 62(2), pp. 59-63. doi: 10.21608/vmjg.2016.363158
VANCOUVER
Fadl, A., A, E., M, A. A., M, G. Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa. Veterinary Medical Journal (Giza), 2016; 62(2): 59-63. doi: 10.21608/vmjg.2016.363158
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