Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014

Document Type : Original Article

Authors

1 Elwatania poultry grandparents, 6october- city stars - Giza, Egypt

2 Department of virology, Faculty of veterinary Medicine, Cairo univveristy,12211 Giza, Egypt.

3 National Laboratory for Veterinary Quality control on poultry Production , Animal Health Reserch Institute, P.O.Box 264- Dokki, Giza-12618, Egypt.

Abstract

In this study, the prevalence of IBV in Egyptian commercial Broiler farms were studied. Samples were collected from flocks in 9 Farms located in 5 governorates (Behira , Giza, Sharkia, Ismailia and Gharbia ) between years 2012 -2014. The birds showed severe respiratory signs with moderate number of chalky white droppings, feed consumption and body weight were markedly reduced. Severe rales with difficult respiration and mortalities were high and ranged from 20% to 25% . Flocks included in this study were vaccinated by classic Massachusetts strain similar commercial live (IB -H120 / MAS) vaccine. 8 out of 9 flocks were positive for IBV using Real time RT PCR. The 500-bp of the SI gene covering the hyper variable region-3 of 5 isolates were amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses closely related to Variant strains of IBV, The viruses were clustered into two groups, the first group included (port said F118-4/2013, Giza F299/2012) which were resemble to IS 1494 Variant 2 strains. The second group include (Behira F161/2012, Sharkia F552/2013 and Ismailia F187/2012) were vaccinal strains resemble D274 vaccine. Monitoring of evolving IBV strains is essential for improving the control of disease in Egypt.

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