Influence of bovine serum albumin supplementation in semen extender on quality of stallion spermatozoa

Document Type : Original Article

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Theriogenology department, Faculty of Veterinary Medicine, Cairo University

Abstract

The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in INRA-82 extender on quality of equine cooled-stored and frozen spermatozoa. Semen was collected from four pure Arab stallions (10 to 12 years old) from Al-Zahraa horse stud on a regular basis (one ejaculate / week) during August through October, 2015. After collection, semen was evaluated using conventional methods. Centrifugation at 600xg for 15 mins, partial removal of seminal plasma leaving 5%, and dilution of pelleted spermatozoa using INRA-82 extender containing different concentrations of BSA (0, 10,15 and 20mg/ml) was carried out. Thenceafter, diluted semen was cooled at 5°C and was kept frozen at -196°C. Evaluation of chilled semen at 5°C revealed that inclusion of INRA-82 extender with BSA at 10, 15, 20 mg/ml diluent showed that, BSA 10mg/ml! was significantly (p < 0.05) higher (80.80%) than the other two concentrations (15, 20mg/ml) of BSA (77.98, 78.30%, respectively) and control (77.88%) in live sperm percent. Evaluation of frozen semen after thawing at 37°C for 30 sec, revealed that BSA 10mg/ml was significantly (p < 0.05) higher in post-thaw motility, viability index and HOST than the other two concentrations and control. In view of the pesent results, it can be concluded that cold-storage (+5° C) and freeze-thaw processing of stallion semen in the presence of BSA antioxidant (10 mg/ml) achieved a significant amelioration in the maintenance of ejaculated sperm quality parameters.

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