Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours

B, Zeidan; El-Sharabassay, A; Dwidar, Y; Ashour, A; Ahmed, E; Arafat, M

doi:10.21608/vmjg.2017.355070

Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours

Document Type : Original Article

Authors

¹ Animal Production Research Institute, Dokki, Giza, Egypt

² Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt

³ Depaartment of Biotechnology, Faculty of Agriculture, Al-Azhar, University, Cairo, Egypt

10.21608/vmjg.2017.355070

Abstract

fifteen male dromedary camels at 5-10 years of age and 500-600 kg live body weighs were used the present study, semen was collected, evaluated and extended with different six extenders (Glucose- yolk- citrate: GYC, Fructose- yolk – citrate: FYC, Lactose -yolk – citrate: LYC, sucrose – Yolk: SYC, Tris-Yolk-Fructose: TYF and Raffinose yolk citrate: RYC) thus final extension rate was 1 semen:4 extender citrate. The extended semen with different extenders (TYP, GYC, PYC, SYC, LYC and RYC) was incubated at 37oC for up to 6 hours. After each incubation time (0 ,1, 2, 4, 5 and 6 hours) the spermatozoa and sperm motility, dead spermatozoa, abnormal spermatozoa and acrosomal damage of spermatozoa where determined sperm penetration into she-camel cervical mucus during incubation at 37oC for up to 4 hours was also estimated. The results revealed that, the extended camel semen with TYF, LYC or SYC or SYC extenders was significantly (P<0.05) higher the percentage of sperm motility, while significantly (P<0.05) lower the percentage of dead spermatozoa abnormal spermatozoa and acrosomal damage of spermatozoa than GYC, FYC and RYC extenders during incubation at 37oC for up to 6 hours. The advancement of incubation time at 37°C decreased significantly (P<0.05) the percentage of motile camel spermatozoa, while significantly (P<0.01) increased the percentage of dead spermatozoa, abnormal spermatozoa and acrosomal damage of spermatozoa with all different extenders (GYC, FYC SYC.LYC, TYP and RYC). The Penetrating ability of the extended camel spermatozoa into she-camel Cervical mucus increased significantly with TYP or LYC extender compared to other extenders during incubation at 37 °C for up to 4 hours. However, the advancement of incubation time at 37oC decreased significantly (P<0.05) the ability to penetrate cervical mucous of camel spermatozoa of the all used extenders.

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