Morphological and Laboratory improvement of Cisplatin - induced azoospermia using adipose derived mesenchymal stem cells in a rabbit model

Document Type : Original Article

Authors

1 Department of Anatomy and embryology, Faculty of Veterinary medicine, Cairo university, Giza,Egypt

2 Department of Histology, Faculty of Veterinary Medicine, Cairo University

3 Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt

4 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

Abstract

The testes are highly susceptible to the adverse effects of chemotherapy and radiation at all stages of life. Exposure to these threats mainly occurs during cancer treatment and as an occupational hazard in radiation centers. The present study investigated the regenerative ability of adipose-derived mesenchymal stem cells (ADMSCs) against the adverse effects of cisplatin on the structure and function of the testes. New Zealand white male rabbits (N = 15) were divided into three groups of five: a negative control group (no treatment), a cisplatin group (single dose of cisplatin into each testis), and a cisplatin with ADMSCs group (cisplatin was injected followed by an ADMSC injection three days later). On day 45 post-treatment, serum testosterone levels were evaluated, and the testes and epididymis were collected for histology, and epididymal sperm analysis. Cisplatin caused damage to the testicular tissue and decreased serum testosterone levels and epididymal sperm counts The ADMSC-treated group displayed a moderate epididymal sperm count, suitable hormone levels, and enhanced testicular tissue morphology. ADMSCs treatment enhanced damaged testicular tissue and modified pathological changes caused by cisplatin.

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