Experimental study on synovial fluid matrix metalloproteinases in dogs affected with osteoarthritis

AbdelHamid, Mohamed Ayad; Ali, Khaled M; Elsayaad, Mohamed E

doi:10.21608/vmjg.2022.267720

Experimental study on synovial fluid matrix metalloproteinases in dogs affected with osteoarthritis

Document Type : Original Article

Authors

Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Egypt

10.21608/vmjg.2022.267720

Abstract

Arthritis is one of the most important diseases causing lameness in dogs as well as other species of animals. Osteoarthritis (OA)cause considerable deterioration of the cartilage and marked enzymatic changes in the synovial fluid, and the joint tissue. The present study was conducted on 25 clinically healthy Mongrel male dogs, with body weight of 15.0 ±1.7kg. Arthritis was induced in 20 dogs and 5 dogs were left as a negative control (normal) group. Osteoarthritis was induced by performing arthrotomy and curettage of the cartilage by creation of two 8.0 mm diameter full thickness chondral defect till exposing the subchondral bone. The dogs were left for 10 days till complete healing of the surgical wound. Amphotericin-B was injected intra-articularly at a dose of 10 mg twice weekly for 2 successive weeks. The dogs were left one week till complete typical symptoms of arthritis were observed. The experimental groups were classified into five groups as control negative (normal), dexamethasone treated, sodium hyaluronate treated, poly sulfated glycosaminoglycan treated and control positive (sham operated). Sensory characteristics of the treated groups were evaluated. Besides, synovial analysis was performed for each animal at the end of the 1st, 2nd, 3rd, and 4th weeks. Matrix metalloproteinase (MMPs) 3 and 13 and tissue inhibitor metalloproteinase (TIMP-1) were detected by ELISA assay. The obtained results revealed that osteoarthritis OA was associated with significant increases in the enzymatic activities of MMPs 3 and 13 and decrease of TIMP-1 as biomarkers of OA. Among the used treatments, polysulfated glucose aminoglycan is the highly recommended therapeutic approach as it caused the highest improvement compared with other treatments.

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