Composing of an oil adjuvant containing BCG as an immunomodulating for rabbit clostridial enterotoxaemia and bloat vaccine

ElHelw, Hamed; Fouad, Shereen M; Mohamed, Marwah M; Mohammed, Gina M; ElKholy, Alaa A.A.

doi:10.21608/vmjg.2022.134349.1010

Composing of an oil adjuvant containing BCG as an immunomodulating for rabbit clostridial enterotoxaemia and bloat vaccine

Document Type : Original Article

Authors

¹ Anaerobic bacterial Vaccine Research Department, VSVRI, Abbassia, Cairo

² Bacterial Diagnostic Products Departement,Vetrinary serum and Vaccine research institute ,Abbasia,Cairo, Agricultural research center

³ Department of Bacterial Diagnostic Products, Veterinary Serum and Vaccine Research Institute, Egypt

⁴ central laboratory for evaluation of veterinary biologics

⁵ Professor of Veterinary Sera and Vaccines Research Institute (VSVRI), Abbassia. Egypt

10.21608/vmjg.2022.134349.1010

Abstract

Clostridium perfringens (C. perfringens) is one of the most important pathogen that is widely distributed in all over the world and responsible for many diseases among life stock. C.perfringens is responsible for toxin production in the intestinal tract of rabbits and that was considered the main cause of enteritis and enterotoxaemia causing high mortality especially at the early weaned rabbits, which lead to high economic losses in rabbits industries. So, this study was aimed to evaluate the ability of oil adjuvant containing sonicated BCG vaccine to increase the immunoresponse for rabbit clostridial enterotoxaemia and bloat vaccine. The study was conducted on twenty New Zealand rabbits were divided in to two groups each of 10 ,Group A(control group) were vaccinated with alpha toxoid adjuvanted with aluminum hydroxide gel at two doses with 21 days apart inoculated subcutaneously , Group B were vaccinated with BCG sonicated Oil adjuvant vaccine of Alpha toxoid of C. perfringens type A, with 2 doses subcutaneously with 21 days. Rabbits in group (B) which vaccinated with BCG sonicated Oil adjuvant vaccine of Alpha toxoid of C. perfringens type A, their serum contain antibody titer of 8 IU/ml( 7.1 IU-8.0 IU/ml) greater than that of control group (A) vaccinated with aluminum hydroxide gel adjuvant vaccine (W=0 , p=0.0001503). From this study, we can concluded that the vaccination of rabbits with oil adjuvant containing sonicated BCG vaccine is effective to increase the immune response against rabbit clostridial enterotoxaemia and bloat vaccine.

Keywords