Fresh versus cryopreserved canine amniotic membrane: Physical and histological comparisons

Document Type : Original Article

Authors

1 Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

2 Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

3 Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

Abstract

The application of amniotic membrane (AM) in clinical practice is expanding all over the world. The most used forms of AM as regenerative scaffold are fresh and cryopreserved amniotic membranes. The present study was designed to compare the physical and histologicalfeatures of fresh and cryopreserved canine amniotic membranes. A total number of three adultfull-term pregnant female mongrel dogs aged approximately 3–5 years and weighing 20–25 kgwere subjected to elective cesarean section to get the amniotic membrane; then the animals AM divided into 2 groups: Group 1 (Amniotic membranes were preserved fresh in a mixture of saline and antibiotic in the refrigerator) and Group 2 (Amniotic membranes were preservedfrozen in -80°c). The present results showed that fresh and frozen canine AM were similar in physical characters such as glistening, tensile strength, thickness, and transparency. The frozen membrane was turbid during thawing at room temperature and then restored its transparency after about 3 minutes. Histological findings of fresh amniotic membrane revealed the presence of all layer epithelium, basement membrane, compact layer, fibroblast layer, and spongy layer in an intactmanner resemble natural histological characters of amniotic membrane. On the other hand, preserved amniotic membrane showing damaged epithelial cells. From the present study, it could be concluded that the method of fresh amniotic membrane storage retained the morphological characteristics (basement membrane and stromal matrix) and epithelial cells viability which is usefully used as a potent source of stem cells and biological scaffold in regenerative therapy as in allograft. Meanwhile, cryopreserved amniotic membrane held its morphological characteristics, it lost the viability of the epithelial cells so it is recommended to use as a biological scaffold in cases with risk of rejection due to antigenic load of the tissueas in xenograft.

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