Trails of use of nano-particles as adjuvant for inactivated bovine viral diarrhea vaccine

Document Type : Original Article

Authors

1 ARC, VSVRI; In House Quality Control Laboratory (IHQCL)

2 VSVRI; Department of Rift Valley Fever Research (DRVFR

3 Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Sueif University

4 VSVRI, Rinderpest Like Diseases Research Department (RPLDRD)

Abstract

Bovine viral diarrhea virus (BVDV) with different genotypes considered as one of the most important viruses in genus Pestivirus at animal level is consider an ideal research model for hepatitis C virus of the same genus of Flavivirus. Many traditional inactivated BVDV vaccine have been used adjuvanted by Alhydragel or oil. The current study focused on formulation anew inactivated BVDV vaccine using innovated nano material (meso porous nano silica) prepared with as 9 parts of viral suspension: 1- Part of dissolved adjuvant. Sterility and safety of the adjuvant alone and with the prepared vaccine containing two genotypes of BVDV (1and 2) and potency test on in vaccinated calves revealed that such adjuvant and vaccine are free from foreign contaminants and the vaccine is potent provided calves with protection against BVDV coverage a period of 9 month for both genotypes using two doses of 1.5mL of both inactivated virus vaccine. This study recommended other complementary study to clarify the detail keeping quality issues and field study.

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