In this work 68 S. aureus isolated frommastitic cows (n=48), camel milk (n=5) and human samples (urine n = 4, abscesses aspiration n = 3, sputum n = 2, nasal swabs n = 2, vaginal swabs n = 1 and wound swabs n = 3) were investigated using phenotypic and molecular identification to detect the occurrence of enterotoxins. Eighteen out of 48 S. aureus isolated from mastitic cows and 5 out of 15 S. aureus isolated from human samples were enterotoxin producers. All toxigenic S. aureus isolated from clinical mastitic cows (13) were toxin type D producer while seb and sed were detected from subclinical mastitic cows. Among human isolates, 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C and D and one isolate produced type C toxin. No toxin was detected from camel isolates. All toxigenic S. aureus isolated from cows (13) were toxin type D producer. Among human isolates 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C & D and one isolate produced type C toxin. Five S. aureuscrude enterotoxin type D were investigated by protein SDS-PAGE. It was clear that all isolates had from 9 to 7 bands. All the isolates had a band at 148-159 kDa, 35-42 kDa, 28-30 kDa, 23-26 kDa, 20-22 kDa and 18-19 kDa. 4 isolates had a band at 48-55 kDa, 3 isolates had a band at 39-42 kDa, 3 isolates had a band at 31-34 kDa and one isolate had a band at 17 kDa.
Mohamed, F. E., H, O., Deif, H., Ragab, E., & Eljakee, J. (2020). Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection. Veterinary Medical Journal (Giza), 66(1), 98-114. doi: 10.21608/vmjg.2020.157909
MLA
Fatma Elzahraa Mohamed; Omnia H; Heba Deif; Eman Ragab; Jakeen Eljakee. "Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection", Veterinary Medical Journal (Giza), 66, 1, 2020, 98-114. doi: 10.21608/vmjg.2020.157909
HARVARD
Mohamed, F. E., H, O., Deif, H., Ragab, E., Eljakee, J. (2020). 'Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection', Veterinary Medical Journal (Giza), 66(1), pp. 98-114. doi: 10.21608/vmjg.2020.157909
VANCOUVER
Mohamed, F. E., H, O., Deif, H., Ragab, E., Eljakee, J. Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection. Veterinary Medical Journal (Giza), 2020; 66(1): 98-114. doi: 10.21608/vmjg.2020.157909
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