The Effect of PRID on the hemodynamics of preovulatory follicular, luteal and the ovarian arteries in Egyptian buffaloes

Document Type : Original Article

Authors

1 Theriogenology Department, Faculty of Veterinary Medicine, Cairo University

2 Animal Reproduction and Artificial Insemination, Veterinary Research Division, National Research Centre, Dokki, Giza

Abstract

The aim of the present investigation was to study the comparative efficacy of two estrous synchronization treatments on follicular, luteal changes, and ovarian hemodynamic associated with hormonal changes of progesterone and estrogen in addition to nitric oxide. Six cyclic buffalo cows were scanned daily along three successive estrous cycles transrectal by Doppler ultrasonography to evaluate the normal ovarian hemodynamic during normal spontaneous ovulation and before the start of experiments. Buffaloes in the current study will be categorized according to the experimental design. The buffalo cows in the first experiment were synchronized with progesterone releasing intra-vaginal device (PRID) - PGF2α (7 days inserted PRID and PGF2α injected on the 6th day, n = 6). In experiment 2 the same animals (n = 6)) were taken a rest for two successive estrous cycles. Then, buffaloes were synchronized with PRID- Modified Co- synch (PRID+GnRH) in which, 7 days inserted PRID intra-virginal then animals will receive 10μg of GnRH at day 0, 250μg of PGF2α at day 7, another 10μg of GnRH administered 48h after the PGF2α injection. Blood sampling and ovarian ultrasound examinations (color and power Doppler modes) were conducted on the day of PRID removal, estrous, and luteal phase. Results revealed that buffaloes treated with PRID - PGF2α had a higher total number of follicles and F1 dominant diameter. Color Doppler ultrasonography revealed that, F1 diameter, F1 area/pixel, F1 antrum area/pixel, F1 granulosa area, and F1 colored area/pixels significantly increased in PRID -PGF2α group as compared to normal spontaneous ovulating and PRID+GnRH group. In addition, the CL diameter and their vascularity (CL Area/pixels, CL Color area/pixels, CL Color area %) were markedly increased in PRID -PGF2α group as compared to normal spontaneous ovulating and PRID+GnRH group concurrent with a high level of plasma estrogen. The higher values of resistance index (RI) of ipsilateral and contralateral ovarian arteries were recorded in PRID -PGF2α and PRID+GnRH compared to the normal spontaneous ovulating. A similar tendency was observed in blood flow volume (BFV). However, the ovarian blood flow indices including time average mean velocity (TAMV) and peak systolic velocity PSV) did not change among different groups. A higher level of plasma estrogen was achieved in PRID -PGF2α as compared to other groups. However, the progesterone levels did not vary among groups. The nitric oxide level was significantly higher in PRID+GnRH as compared to others. In conclusion, synchronization with the PRID -PGF2α protocol could be enhanced the follicular activity and hemodynamics of Egyptian buffaloes.

Keywords

Files

Share

How to cite

Statistics